Enzyme Immunoassay of Monensin Using Peroxidase as Label
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منابع مشابه
DETERMINATION OF SERUM CORTISOL LEVEL BY A DIREC T ENZYME IMMUNOASSAY USING PENICILLINASE AS LABEL
Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...
متن کاملdetermination of serum cortisol level by a direc t enzyme immunoassay using penicillinase as label
serum cortisol level was measured by an enzyme immunoassay (eia) using the enzyme penicillinase as a label without prior extraction and purification. polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-cmo) conjugated to bovine serum albumin (bsa). this antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticosteron...
متن کاملENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملA Heterologous Enzyme Linked Immunosorbant Assay of Morphine Using Penicillinase as Label
A rapid, sensitive, specific and high through-put enzyme-linked immunosorbant assay (ELISA) methodfor determination of morphine in urine samples using penicillinase as label enzyme has been developed. Noextraction or chromatography was included in this assay procedure. Immunoglobulin (Ig) purified polyclonalanti-bodies against a C6-hemisuccinate derivative of morphine (M-C6-HS...
متن کاملEnzyme-Linked Immunosorbent Assay of Progesterone in Serum Using Penicillinase as Label
An enzyme-linked immunosorbent assay (ELISA) for progesterone measurement in serum or plasma samples using penicillinase as label enzyme is reported. A C3 and C11 derivatives of progesterone were prepared and conjugated to bovin serum albumin (BSA). Polyclonal antibody against these two immunogens were prepared in New Zealand white rabbits. Purified Ig fractions of antibodies were immobilized o...
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ژورنال
عنوان ژورنال: Eisei kagaku
سال: 1984
ISSN: 0013-273X
DOI: 10.1248/jhs1956.30.4_243